DNA

Part:BBa_J119003:Design

Designed by: Caleb J. Carr   Group: Eckdahl_Lab   (2011-07-21)

BsmBI-Rbs-LacZ1-BsaI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1632
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1632
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1632
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1632
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1626


Design Notes

This is NOT a BioBrick part. It is only functional if properly re-assembled with J11904. Can be used in combination with other split-genes to solve HPP (Hamiltonian Path Problems).

Reporting of this gene causes a blue color to become present when the E Coli are exposed to X-Gal. However, it is possible to have an Alpha-Mutation which causes the first half of the gene (independent of the second half) to report with blue coloring. Careful attention must be paid to be sure of a correct result instead of a false positive.

Source

PCR amplification of LacZ.

References